CRISPR-Cas12a-Based Biosensing Platform for Rapid Detection of Foodborne Salmonella Contamination
DOI:
https://doi.org/10.5281/zenodo.22638243Keywords:
CRISPR-Cas12a, Salmonella, foodborne pathogen detection, biosensor, lateral flow assayAbstract
Background: Nontyphoidal Salmonella is a leading cause of foodborne illness, and culture-based confirmation is too slow for
modern supply-chain decisions. Objective: To synthesise published evidence on CRISPR-Cas12a (and related Cas13/Cas9-family)
diagnostic platforms for detecting Salmonella and other foodborne pathogens. Methods: Narrative review of peer-reviewed
studies identified via PubMed, Google Scholar and Crossref using terms combining "CRISPR," "Cas12a," "Cas13," "Salmonella,"
"foodborne," "RPA/LAMP," and "lateral flow." Findings: Amplification-coupled Cas12a assays (RPA-, LAMP- or RAA-CRISPR)
reliably reach limits of detection of 1-100 CFU/mL within 30-90 minutes, with fluorescent, colorimetric, lateral-flow and
glucose-meter readouts demonstrated for Salmonella; amplification-free formats trade sensitivity for speed. Conclusions:
CRISPR-Cas12a platforms now approach performance suitable for field deployment, though standardisation and multiplexing
remain barriers to routine adoption.
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